Novel amplification of DNA in a hairpin structure: towards a radical elimination of PCR errors from amplified DNA.

@article{Kaur2003NovelAO,
  title={Novel amplification of DNA in a hairpin structure: towards a radical elimination of PCR errors from amplified DNA.},
  author={Manjit Kaur and G. Mike Makrigiorgos},
  journal={Nucleic acids research},
  year={2003},
  volume={31 6},
  pages={e26}
}
Errors introduced during PCR amplification set a selectivity limit for microsatellite analysis and molecular mutation detection methods since polymerase misincorporations invariably get confused with genuine mutations. Here we present hairpin-PCR, a new form of PCR that completely separates genuine mutations from polymerase misincorporations. Hairpin-PCR operates by converting a DNA sequence to a hairpin following ligation of oligonucleotide caps to DNA ends. We developed conditions that allow… CONTINUE READING