Novel amidinating cross-linker for facilitating analyses of protein structures and interactions.

@article{Lauber2010NovelAC,
  title={Novel amidinating cross-linker for facilitating analyses of protein structures and interactions.},
  author={Matthew A. Lauber and James Patrick Reilly},
  journal={Analytical chemistry},
  year={2010},
  volume={82 18},
  pages={7736-43}
}
A novel bifunctional thioimidate cross-linking reagent (diethyl suberthioimidate) that modifies amines without sacrificing their native basicity is developed. Intermolecular cross-linking of neurotensin and intramolecular cross-linking of cytochrome c under physiological conditions is investigated with this reagent. Because it does not perturb the electrostatic properties of a protein, it is unlikely to lead to artifactual conclusions about native protein structure. The interpeptide cross-links… CONTINUE READING