Novel T7-like expression systems used for Halomonas.

@article{Zhao2017NovelTE,
  title={Novel T7-like expression systems used for Halomonas.},
  author={Han Zhao and Haoqian M Zhang and Xiangbin Chen and Teng Li and Qiong Wu and Qi Ouyang and Guoqiang Chen},
  journal={Metabolic engineering},
  year={2017},
  volume={39},
  pages={
          128-140
        }
}
Construction of T7-Like Expression System in Pseudomonas putida KT2440 to Enhance the Heterologous Expression Level
TLDR
The constructed and optimized the T7-like and T7 expression system in P. putida, thus providing a set of applicable chassis and corresponding plasmids to improve recombinant expression level, expecting to be used for difficult-to-express proteins.
CRISPR/Cas9 editing genome of extremophile Halomonas spp.
Identification and validation of four novel promoters for gene engineering with broad suitability across species.
TLDR
This study demonstrates an example of applying bioinformatics to find new biological components for gene operation and provides four novel promoters with broad suitability, which enriches the usable range of promoters to realize accurate regulation in different genetic backgrounds.
New Insight into Plasmid-Driven T7 RNA Polymerase (PDT7) in Escherichia coli and Used as Genetic Amplifier for Biosensor.
TLDR
The T7 toxicity was elucidated from the construction processing and cell growth characterization, showing the importance of optimal orthogonality for PDT7, and the orthogonal effects in toxicity and instability were investigated.
Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis.
TLDR
This study reports an approach to construct a promoter library based on the Pporin core region, namely, from the -35 box to the transcription start site, a spacer and an insulator, suitable for the transcription of many different heterologous genes.
Halomonas as a chassis
TLDR
Increasing efforts are made to develop their versatile cell factories powered by synthetic biology to demonstrate a new biomanufacturing strategy under open and continuous processes with significant cost-reduction on process complexity, energy, substrates and fresh water consumption.
Construction of Halomonas bluephagenesis capable of high cell density growth for efficient PHA production
TLDR
A high-cell-density H. bluephagenesis TDHCD-R3 obtained after 3 rounds of selection showed an obvious enhancement of resistance to toxic metabolites including acetate, formate, lactate and ethanol compared to wild-type.
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References

SHOWING 1-10 OF 55 REFERENCES
Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp.
TLDR
An inducible expression system was constructed in phaC-deleted Halomonas TD01 by integrating the lac repressor gene into the porin site in the host chromosome, and the stable and strong chromosomal expression method in HalomonAs TD spp.
Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp.
TLDR
The constitutive and inducible promoters were employed to regulate the biosynthetic pathway of poly‐3‐hydroxybutyrate (PHB) in Halomonas TD01, demonstrating the usefulness of the expression systems.
Engineering of synthetic, stress-responsive yeast promoters
TLDR
A set of strong, synthetic promoters for budding yeast Saccharomyces cerevisiae are engineered and characterized that are inducible under acidic conditions (pH ≤ 3) and improved the low-pH performance of the YGP1 promoter by modifying transcription factor binding sites in its upstream activation sequence.
Tunable switch mediated shikimate biosynthesis in an engineered non-auxotrophic Escherichia coli
TLDR
The tunable switch developed in this study is an efficient tool for regulating indispensable genes involved in critical metabolic pathways that can conditionally decrease gene expression and substituted it for the original aroK promoters.
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol
TLDR
Saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms were substituted for Clostridial enzymes and their effect on n- butanol production was compared.
Genome-engineered Sinorhizobium meliloti for the production of poly(lactic-co-3-hydroxybutyric) acid copolymer.
TLDR
The chromosome integrant strain, with the introduced genes under the control of the native phbC promoter, is able to produce over 15% cell dry mass of poly(lactate-co-3-hydroxybutyrate), containing 30 mol% lactate, from growth on mannitol, the first demonstration of production of this copolymer in the Alphaproteobacteria.
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