Novel Symmetric and Asymmetric DNA Scission Determinants for Streptococcus pneumoniae Topoisomerase IV and Gyrase Are Clustered at the DNA Breakage Site*

@article{Leo2005NovelSA,
  title={Novel Symmetric and Asymmetric DNA Scission Determinants for Streptococcus pneumoniae Topoisomerase IV and Gyrase Are Clustered at the DNA Breakage Site*},
  author={Elisabetta Leo and Katherine A. Gould and X S Pan and Giovanni Capranico and Mark R Sanderson and Manlio Palumbo and L Mark Fisher},
  journal={Journal of Biological Chemistry},
  year={2005},
  volume={280},
  pages={14252 - 14263}
}
Topoisomerase (topo) IV and gyrase are bacterial type IIA DNA topoisomerases essential for DNA replication and chromosome segregation that act via a transient double-stranded DNA break involving a covalent enzyme-DNA “cleavage complex.” Despite their mechanistic importance, the DNA breakage determinants are not understood for any bacterial type II enzyme. We investigated DNA cleavage by Streptococcus pneumoniae topo IV and gyrase stabilized by gemifloxacin and other antipneumococcal… Expand
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