Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering

@inproceedings{Derntl2015NovelSF,
  title={Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering},
  author={Christian G. Derntl and Daniel P. Kiesenhofer and Robert L. Mach and Astrid R. Mach-Aigner and Dan Cullen},
  booktitle={Applied and environmental microbiology},
  year={2015}
}
The state-of-the-art procedure for gene insertions into Trichoderma reesei is a cotransformation of two plasmids, one bearing the gene of interest and the other a marker gene. This procedure yields up to 80% transformation efficiency, but both the number of integrated copies and the loci of insertion are unpredictable. This can lead to tremendous pleiotropic effects. This study describes the development of a novel transformation system for site-directed gene insertion based on auxotrophic… CONTINUE READING
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