Novel PCR assay for determining the genetic sex of mice.

  title={Novel PCR assay for determining the genetic sex of mice.},
  author={Lindsey McFarlane and Vy Truong and James S. Palmer and Dagmar Wilhelm},
  journal={Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation},
  volume={7 4},
A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable… CONTINUE READING

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