Novel DNA assay for cytomegalovirus detection: comparison with conventional culture and pp65 antigenemia assay

@article{Veal1996NovelDA,
  title={Novel DNA assay for cytomegalovirus detection: comparison with conventional culture and pp65 antigenemia assay},
  author={Nary Veal and Christopher Payan and D Fray and L Sarol and Odile Blanchet and S. Kouyoumdjian and Françoise Lunel},
  journal={Journal of Clinical Microbiology},
  year={1996},
  volume={34},
  pages={3097 - 3100}
}
We compared conventional cytomegalovirus (CMV) isolation, rapid viral culture, a CMV pp65 antigenemia assay, and a novel CMV DNA hybrid capture system (HCS). A total of 309 blood samples from individuals in different risk groups were assessed by at least two of the methods mentioned above. Leukocytes were recovered either after centrifugation in Leucosep tubes containing 1.080 Ficoll for pp65 assay or after simple differential lysis steps for DNA detection. HCS was based on DNA hybridization… 
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Multicenter Comparison of the Digene Hybrid Capture CMV DNA Assay (Version 2.0), the pp65 Antigenemia Assay, and Cell Culture for Detection of Cytomegalovirus Viremia
TLDR
The high sensitivity and specificity of the Hybrid Capture assay along with its simplicity and flexibility make it a clinically useful assay for the detection of CMV viremia in immunocompromised or immunosuppressed patients.
Quantitative analysis of cytomegalovirus load using a real‐time PCR assay
TLDR
This assay is sensitive and useful for estimating the CMV genome load not only in peripheral blood leukocytes but also in plasma, and can be very helpful for diagnosing CMV‐related diseases and monitoring the virus load in patients with CMV infections.
Evaluation of Murex CMV DNA Hybrid Capture Assay for Detection and Quantitation of Cytomegalovirus Infection in Patients following Allogeneic Stem Cell Transplantation
TLDR
All patients withCMV disease were correctly identified by HCS; however, the lower sensitivity limit of the HCS assay may still be insufficient to allow diagnosis of CMV infection early enough to prevent CMV disease in patients following allogeneic SCT.
Comparative quantification of human cytomegalovirus DNA in blood of immunocompromised patients by PCR and Murex Hybrid Capture System.
Evaluation of the Murex Hybrid Capture Cytomegalovirus DNA Assay versus Plasma PCR and Shell Vial Assay for Diagnosis of Human Cytomegalovirus Viremia in Immunocompromised Patients
TLDR
HCA and plasma PCR were more consistently positive than SVA during viremic episodes, and the use of HCA or plasma PCR may improve the diagnosis and management of CMV disease in susceptible patient groups.
Quantitation of Cytomegalovirus: Methodologic Aspects and Clinical Applications
SUMMARY Cytomegalovirus (CMV) is an important pathogen in transplant recipients and human immunodeficiency virus (HIV)-infected individuals. Major progress has been made in developing quantitative…
Human cytomegalovirus DNA kinetics using a novel HCMV DNA quantitative assay in white blood cells of immunocompromised patients under Ganciclovir therapy.
Comparison of cytomegalovirus pp‐65 antigenemia assay and plasma DNA correlation with the clinical outcome in transplant recipients
  • M. Oña, S. Melón, E. Gómez
  • Medicine, Biology
    Transplant international : official journal of the European Society for Organ Transplantation
  • 2005
TLDR
In nine patients, maximum antigenemia coincided with highest plasma DNA viral load, but in 10 highest DNAemia occurred 6 days later, while on the contrary, antigenemia declined faster than CMV‐DNAnemia, after treatment.
Determination of human cytomegalovirus pp65 antigenemia among renal transplant patients
TLDR
A pp65 count of 23 and above was considered significant in clinical settings since it was found that these clinical specimens yield positive culture result and a mean of 23 cells was determined to yield a positive isolation of HCMV.
Significance of leukocyte concentration in the performance of the quantitative cytomegalovirus (CMV) antigenemia assay.
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References

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