Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies

@inproceedings{Shen2010NovelCA,
  title={Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies},
  author={Dee Shen and Jack Coleman and Eric Lap-Shing Chan and Thomas Peter Nicholson and Lijun Dai and Paul W. Sheppard and Wayne F. Patton},
  booktitle={Cell Biochemistry and Biophysics},
  year={2010}
}
Aggresomes and related inclusion bodies appear to serve as storage depots for misfolded and aggregated proteins within cells, which can potentially be degraded by the autophagy pathway. A homogenous fluorescence-based assay was devised to detect aggregated proteins inside aggresomes and inclusion bodies within an authentic cellular context. The assay employs a novel red fluorescent molecular rotor dye, which is essentially nonfluorescent until it binds to structural features associated with the… CONTINUE READING
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