Novel Analytic Criteria and Effective Plate Designs for Quality Control in Genome-Scale RNAi Screens

@article{Zhang2008NovelAC,
  title={Novel Analytic Criteria and Effective Plate Designs for Quality Control in Genome-Scale RNAi Screens},
  author={Xiaohua Douglas Zhang},
  journal={Journal of Biomolecular Screening},
  year={2008},
  volume={13},
  pages={363 - 377}
}
One of the most fundamental challenges in genome-wide RNA interference (RNAi) screens is to glean biological significance from mounds of data, which relies on the development and adoption of appropriate analytic methods and designs for quality control (QC) and hit selection. Currently, a Z-factor-based QC criterion is widely used to evaluate data quality. However, this criterion cannot take into account the fact that different positive controls may have different effect sizes and leads to… 

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