Novel β-Secretase Cleavage of β-Amyloid Precursor Protein in the Endoplasmic Reticulum/Intermediate Compartment of NT2N Cells

@article{Chyung1997NovelC,
  title={Novel β-Secretase Cleavage of β-Amyloid Precursor Protein in the Endoplasmic Reticulum/Intermediate Compartment of NT2N Cells },
  author={Abraham S. C. Chyung and Barry D. Greenberg and David G. Cook and Robert W. Doms and Virginia M. Y. Lee},
  journal={The Journal of Cell Biology},
  year={1997},
  volume={138},
  pages={671 - 680}
}
Previous studies have demonstrated that NT2N neurons derived from a human embryonal carcinoma cell line (NT2) constitutively process the endogenous wild-type beta-amyloid precursor protein (APP) to amyloid beta peptide in an intracellular compartment. These studies indicate that other proteolytic fragments generated by intracellular processing must also be present in these cells. Here we show that the NH2-terminal fragment of APP generated by beta-secretase cleavage (APPbeta) is indeed produced… CONTINUE READING