Novel, versatile, and tightly regulated expression system for Escherichia coli strains.

@article{Choi2010NovelVA,
  title={Novel, versatile, and tightly regulated expression system for Escherichia coli strains.},
  author={Young Jin Choi and Lyne Morel and Teffanie Le François and Denis Bourque and Lucie Bourget and Denis Groleau and Bernard Massie and Carlos B. Miguez},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 15},
  pages={5058-66}
}
A novel tightly regulated gene expression system was developed for Escherichia coli by applying the regulatory elements of the Pseudomonas putida F1 cym and cmt operons to control target gene expression at the transcriptional level by using p-isopropylbenzoate (cumate) as an inducer. This novel expression system, referred to as the cumate gene switch, includes a specific expression vector, pNEW, that contains a partial T5 phage promoter combined with the Pseudomonas-based synthetic operator and… CONTINUE READING

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