Novel, ultrasensitive, Q-beta replicase-amplified hybridization assay for detection of Chlamydia trachomatis.

@article{Shah1994NovelUQ,
  title={Novel, ultrasensitive, Q-beta replicase-amplified hybridization assay for detection of Chlamydia trachomatis.},
  author={Jahngir Alam James F. Kile Donald Little Khaled Mohamed Sa Shah and Jun Liu and Jocelyn Smith and Steven N. Popoff and Gail Radcliffe and William J. O'Brien and G Serpe and Dani{\`e}le Olive and W. O. Redman King},
  journal={Journal of clinical microbiology},
  year={1994},
  volume={32 11},
  pages={2718-24}
}
A sensitive, nonisotopic hybridization assay termed "dual capture" is described. The assay rapidly and specifically detects very low levels of target nucleic acids and organisms. The assay is based on the principles of sandwich hybridization, reversible target capture, and Q-Beta replicase amplification. The assay can be completed in less than 4 h, and in the described model format, it detects Chlamydia trachomatis rRNA or rDNA. Up to 96 samples can be analyzed simultaneously. The assay employs… CONTINUE READING