Novel, Oxygen-Insensitive Group 5 [NiFe]-Hydrogenase in Ralstonia eutropha

  title={Novel, Oxygen-Insensitive Group 5 [NiFe]-Hydrogenase in Ralstonia eutropha},
  author={Caspar Sch{\"a}fer and B. Friedrich and O. Lenz},
  journal={Applied and Environmental Microbiology},
  pages={5137 - 5145}
  • Caspar Schäfer, B. Friedrich, O. Lenz
  • Published 2013
  • Chemistry, Medicine
  • Applied and Environmental Microbiology
  • ABSTRACT Recently, a novel group of [NiFe]-hydrogenases has been defined that appear to have a great impact in the global hydrogen cycle. This so-called group 5 [NiFe]-hydrogenase is widespread in soil-living actinobacteria and can oxidize molecular hydrogen at atmospheric levels, which suggests a high affinity of the enzyme toward H2. Here, we provide a biochemical characterization of a group 5 hydrogenase from the betaproteobacterium Ralstonia eutropha H16. The hydrogenase was designated an… CONTINUE READING
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