Notes on the characterization of prokaryote strains for taxonomic purposes.

  title={Notes on the characterization of prokaryote strains for taxonomic purposes.},
  author={Brian J. Tindall and Ramon Rossell{\'o}-M{\'o}ra and Hans-J{\"u}rgen Busse and Wolfgang Ludwig and Peter K{\"a}mpfer},
  journal={International journal of systematic and evolutionary microbiology},
  volume={60 Pt 1},
Taxonomy relies on three key elements: characterization, classification and nomenclature. All three elements are dynamic fields, but each step depends on the one which precedes it. Thus, the nomenclature of a group of organisms depends on the way they are classified, and the classification (among other elements) depends on the information gathered as a result of characterization. While nomenclature is governed by the Bacteriological Code, the classification and characterization of prokaryotes… 

Systematics of prokaryotes: the state of the art

The term polyphasic taxonomy, which was coined almost 40 years ago and aimed at the integration of many levels of information (from molecular to ecological data) thereby allowing a more holistic view, should be revisited in the light of the enormous potential of the novel information associated with large data sets.

Prokaryotic taxonomy in the sequencing era and the role of MLSA in classification

There appears to be a tendency to classify new species on the basis of comparative sequence analyses of 16S rRNA gene sequences and other gene sequence data (multilocus sequence analyses, MLSA), contrary to the indications of other data.

Taxonomy and Classification of Bacteria

This chapter discusses the state of the art in bacterial taxonomy. It addresses criteria for species delineation, the polyphasic species concept, and multilocus sequence- and whole-genome

Advantages and limitations of genomics in prokaryotic taxonomy.

  • E. SentausaP. Fournier
  • Biology
    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2013
Genomics has reached a stage where it may be used in prokaryotic taxonomic classification, with criteria such as the genome index of average nucleotide identity being an alternative to DNA-DNA hybridization.

Time to revisit polyphasic taxonomy

Bacterial taxonomists must urgently reconsider how to describe and name novel bacteria in the genomic era, and should consider using a full genome sequence and a minimal description of phenotypic characteristics as a basic, sufficient, cost-effective and appropriate biological identity card for a species description.

Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy †

Students isolate a strain of Pseudomonas from soil and characterize its biochemical and physiological properties, as well as determine the DNA sequence of its 16S rRNA genes.



Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae.

The main criterion for the classification of a novel species is that it forms a monophyletic group based on 16S rRNA gene sequence-based phylogenetic analysis, however, some groups might also include closely related species.

Progress and problems in bacterial systematics

It is felt that bacterial systematics could be placed on a firm foundation and the following steps were taken to revise the Bacteriological Code so that in order for a name to be validly published a prescribed minimal description would be required.

Taxonomic notes: a proposal for recording the properties of putative taxa of procaryotes.

This note is presented in advance of discussions by the International Committee on Systematic Bacteriology at the forthcoming congress (July 1994) so that there may be discussions and reaction by colleagues.

Molecular Biology and Bacterial Phylogeny

The evolutionary tree of bacteria remains largely invisible, if one uses only phenotypic features.

Proposal of Minimal Standards for Describing New Taxa of Methanogenic Bacteria

These requirements, as well as methods for determining specified characteristics, are indicated here and are proposed as minimal standards for the taxonomic description of new taxa of methanogens.

3 Enzyme Tests in Bacterial Identification

Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes).

Revised standards for novel species of the class Mollicutes are proposed that reflect recent advances in molecular systematics and the species concept for prokaryotes.

Recommended minimal standards for describing new taxa of the family Halomonadaceae.

The minimal standards are considered as guidelines for authors to prepare descriptions of novel taxa and have been endorsed by the International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Halomonadaceae.

Valid publication of names of prokaryotes according to the rules of nomenclature: past history and current practice.

There is a need to review both the historical aspects and current practices so that the current generation of prokaryote systematists have an up-to-date overview of some of the key elements.

Deoxyribonucleic Acid Reassociation in the Taxonomy of Enteric Bacteria

The application and utility of DNA relatedness to bacterial taxonomy are discussed in order to stimulate greater awareness and use of this technique.