Notes on Selling'S Green-Light Method for Critical Microscopy (Continued)

@inproceedings{WatermanNotesOS,
  title={Notes on Selling'S Green-Light Method for Critical Microscopy (Continued)},
  author={Henry C. Waterman and H. E. Warmke}
}
Kill root tips in 1 part glacial acetic acid to 3 parts absolute alcohol for 12 or more hours. Remove from killing fluid and place for 5 to 10 minutes in a solution consisting of 1 part 95% alcohol to 1 part concentrated HCl. Transfer to Carnoy's fluid for 5 minutes or longer. Cut a small piece (0.5 mm. or less) off the tip of the root and place on a clean slide in a small drop of iron-aceto-carmin stein. Press directly on the piece of root with a small flat scalpel; the cells will now separate… CONTINUE READING