Notch-1 activation by familial Alzheimer's disease (FAD)-linked mutant forms of presenilin-1.

@article{Nakajima2000Notch1AB,
  title={Notch-1 activation by familial Alzheimer's disease (FAD)-linked mutant forms of presenilin-1.},
  author={Mitsunari Nakajima and Takahiko Shimizu and Takuji Shirasawa},
  journal={Journal of neuroscience research},
  year={2000},
  volume={62 2},
  pages={311-7}
}
We prepared a cleavage site-directed antibody against Notch-1, that specifically recognized the cleaved Notch-1 intracellular domain (NICD). To assess Notch-1 processing and its nuclear localization in familial Alzheimer's disease (FAD)-linked presenilin-1 (PS-1) mutants, we overexpressed wild type, M146V, A246E, C410Y, or deltaE9 PS-1 mutant with a membrane-bound Notch-1 in a PS-1-deficient cell line. On Western blot and immunocytochemical analyses using the NICD specific antibody, M146V and… CONTINUE READING

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