Norway spruce somatic embryogenesis: high-frequency initiation from light-cultured mature embryos

@article{Verhagen2004NorwaySS,
  title={Norway spruce somatic embryogenesis: high-frequency initiation from light-cultured mature embryos},
  author={Shirley A. Verhagen and Steven R. Wann},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={16},
  pages={103-111}
}
Somatic embryos and rooted plantlets have been regenerated from light-initiated embryogenic callus derived from mature embryos of Picea abies. Under a 16 h photoperiod, mature zygotic embryos were cultured on a modified half-strength Murashige & Skoog medium without NH4NO3 and supplemented with 5 mM glutamine, 4.5 μM N6-benzyladenine and 10.7 μM naphthaleneacetic acid or 10 μM 2,4-dichlorophenoxyacetic acid. White translucent embryogenic callus, proliferating from the callusing hypocotyl region… CONTINUE READING
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