Northern hybridization: rapid and simple electrophoretic conditions.

  title={Northern hybridization: rapid and simple electrophoretic conditions.},
  author={Roger Pell{\'e} and Noel B Murphy},
  journal={Nucleic acids research},
  volume={21 11},
Current methods for Northern blot analysis to determine mRNA abundance in tissues are tedious and utilize toxic chemicals. The requirement for chemicals of high purity, free of ribonucleases, and the lability of RNA, in comparison to DNA, are additional factors preventing wider use of the technique. For Northern blot analysis, RNA is generally denatured with glyoxal (1) or formaldehyde (2) prior to electrophoresis in agarose gels. The gels require extensive washing for ethidium bromide or… CONTINUE READING
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