Northern blot analysis of simple repetitive sequence transcription in plants

  title={Northern blot analysis of simple repetitive sequence transcription in plants},
  author={Gotz Gortner and Markus Pfenninger and G{\"u}nter Kahl and Kurt Weising},
The presence of simple repetitive sequence motifs in RNA from various plant species was probed by Northern blot analysis. Hybridization of total, poly(A)+‐ and poly(A)−‐RNA with microsatellite‐complementary oligonucleotide probes revealed distinct bands with most but not all probe/species combinations, demonstrating the presence of di‐, tri‐ and tetranucleotide repeat motifs in plant transcripts. Only trinucleotide repeat‐derived hybridization signals were found to be enriched in the poly(A… 

Genetic basis of host - specificity in Sclerospora graminicola, the pearl millet downy mildew pathogen

The genetic basis of host specificity in Sclerospora graminicola, the causal agent of downy mildew in pearl millet, was studied in a host-pathogen cross-inoculation experiment and the change in virulence in Path-1 and its' adaptation to the new host, 700651, was demonstrated.

Evolution and the genetic analysis of populations: 1950-2000

The accumulated new insights of the last 50 years show that the pervasive influence of historical contingency has non-trivial consequences for evolutionary biology and taxonomy.



Non-Alu family interspersed repeats in human DNA and their transcriptional activity.

Most of the highly repetitive (greater than 50,000 copies) human interspersed repeats have already been identified, and the bulk of the in vivo transcriptional activity of these repeats appears to be nonspecific read through from other promoters.

A transcriptional repressor obtained by alternative translation of a trinucleotide repeat

The translational reading frame of the highly polymorphic CAG-repeat that normally encodes poly-Gln in the N-terminal portion of the rat glucocorticoid receptor is changed and it is shown that GR desactivation is obtained only when a very precise threshold length of the repeat is reached.

PCR-amplified microsatellites as markers in plant genetics.

Polymerase chain reaction amplification of ( AT)n and (TAT)n microsatellites in soybean revealed that they are highly polymorphic, as a consequence of length variation, somatically stable and inherited in a co-dominant Mendelian manner.

On simple repeated GATCA sequences in animal genomes: a critical reappraisal.

The exact provenance of GATCA sequences in present-day eukaryotes cannot be pinpointed, but evolutionary conservation and several modes of de novo generation are discussed.

Enhanced gene expression by the poly(dT-dG).poly(dC-dA) sequence

The TG-element appeared to have characteristics similar to those of viral enhancers, but its enhancer-like activity was much weaker than that of the simian virus 40 enhancer, and, unlike many viral enhancies, it was equally active in monkey and in human cells.

Oligonucleotide fingerprinting using simple repeat motifs: a convenient, ubiquitously applicable method to detect hypervariability for multiple purposes.

It is suggested here that due to the ubiquitous interspersion of their targets, short probes for simple repeat sequences are especially useful tools for ordering genomic cosmid, yeast artificial chromosome and phage banks.

Purification of mRNA directly from crude plant tissues in 15 minutes using magnetic oligo dT microspheres.

Using this method poly A+ RNA can be prepared successfully from several tissues from barley such as embryo, aleurone, seedlings, roots and leaves and can be used without further purification for Northern hybridization, in vitro translation and cDNA synthesis.

(CT)n (GA)n repeats and heat shock elements have distinct roles in chromatin structure and transcriptional activation of the Drosophila hsp26 gene

The results suggest that GAGA factor, an abundant protein factor required for normal expression of many Drosophila genes, and heat shock factor, a specific transcription factor activated upon heat shock, play distinct roles in gene regulation: the G AGA factor establishes and/or maintains the DH sites prior to heat shock induction, while the activated heatshock factor recognizes and binds HSEs located within theDH sites to trigger transcription.