Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues

  title={Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues},
  author={Sylvia Streit and Christoph W Michalski and Mert Erkan and J{\"o}rg Kleeff and Helmut Friess},
  journal={Nature Protocols},
Investigation of gene expression significantly contributes to our knowledge of the regulation and function of genes in many areas of biology. In this protocol, we describe how northern blot analysis is used to identify gene expression patterns at the RNA level in human cancer cells as well as in cancerous and normal tissues. RNA molecules are separated by gel electrophoresis and are subsequently transferred to a porous membrane by capillary action. Specific sequences in the RNA are detected on… 

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