Norovirus mixed infection in an oyster-associated outbreak: an opportunity for recombination

@article{Symes2007NorovirusMI,
  title={Norovirus mixed infection in an oyster-associated outbreak: an opportunity for recombination},
  author={Sally J Symes and Ishara C. Gunesekere and J. Andrew Marshall and Peter J. Wright},
  journal={Archives of Virology},
  year={2007},
  volume={152},
  pages={1075-1086}
}
We describe an outbreak of gastroenteritis in which the nucleic acid of three distinct noroviruses was amplified from the same fecal sample. To enable the separate amplification of each virus, an inclusion/exclusion RT-PCR primer design strategy was developed. This paired a virus-specific exclusion primer (designed with the exact sequence of one virus in a region displaying low conservation among the three viruses) with a virus-nonspecific inclusion primer (designed in a conserved region). Thus… CONTINUE READING

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