Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon.

@article{Dydensborg2006NormalizingGF,
  title={Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon.},
  author={Anders Bondo Dydensborg and Elizabeth Herring and Jo{\"e}lle Auclair and Eric A Tremblay and Jean-François Beaulieu},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  year={2006},
  volume={290 5},
  pages={
          G1067-74
        }
}
As for other mRNA measurement methods, quantitative RT-PCR results need to be normalized relative to stably expressed genes. Widely used normalizing genes include beta-actin and glyceraldehyde-3-phosphate dehydrogenase. It has, however, become clear that these and other normalizing genes can display modulated patterns of expression across tissue types and during complex cellular processes such as cell differentiation and cancer progression. Our objective was to set the basis for identifying… 
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