Normalized quantification by real-time PCR of Epstein-Barr virus load in patients at risk for posttransplant lymphoproliferative disorders.

@article{Jabs2001NormalizedQB,
  title={Normalized quantification by real-time PCR of Epstein-Barr virus load in patients at risk for posttransplant lymphoproliferative disorders.},
  author={Wolfram J. Jabs and Holger Hennig and Michael A. Kittel and Klaus Pethig and Françoise Smets and Peter Bucsky and Holger Kirchner and H J Wagner},
  journal={Journal of clinical microbiology},
  year={2001},
  volume={39 2},
  pages={564-9}
}
The load of Epstein-Barr virus (EBV) in peripheral blood mononuclear cells of transplant recipients represents a predictive parameter for posttransplant lymphoproliferative disorders (PTLD). The aim of our work was to develop a rapid and reliable PCR protocol for the quantification of cell-associated EBV DNA in transplant recipients. In contrast to previous studies, a protocol that facilitated quantification independent of photometric nucleic acid analysis was established. We took advantage of… CONTINUE READING