Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice

@article{Kim2003NormalizationOR,
  title={Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice},
  author={Bo-Ra Kim and H Nam and Soo-Un Kim and Su-il Kim and Yung-Jin Chang},
  journal={Biotechnology Letters},
  year={2003},
  volume={25},
  pages={1869-1872}
}
Reverse transcription followed by real-time quantitative polymerase chain reaction (RT Q-PCR) is useful for the systematic measurement of plant physiological changes in gene expression. The validity of using 18S rRNA and three housekeeping genes, glyceraldehyde-3-phosphate dehydrogenase, actin, and tubulin, was tested as a reference of RT Q-PCR. Under various growth stages of etiolated seedlings, different cultivars, and various times after UV-irradiation treatment, expression level of 18S rRNA… CONTINUE READING
145 Citations
16 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 145 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 16 references

Accurate normalization

  • A de Paepe, F Speleman
  • 2002

Quant - itative PCR by continuous fluorescence monitoring of a double strand DNA specific binding dye

  • R Rasmussen, T Morrison, M Herrmann, C Wittwer
  • Biochemica
  • 1998
1 Excerpt

Similar Papers

Loading similar papers…