Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice

  title={Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice},
  author={Bo-Ra Kim and H Nam and Soo-Un Kim and Su-il Kim and Yung-Jin Chang},
  journal={Biotechnology Letters},
Reverse transcription followed by real-time quantitative polymerase chain reaction (RT Q-PCR) is useful for the systematic measurement of plant physiological changes in gene expression. The validity of using 18S rRNA and three housekeeping genes, glyceraldehyde-3-phosphate dehydrogenase, actin, and tubulin, was tested as a reference of RT Q-PCR. Under various growth stages of etiolated seedlings, different cultivars, and various times after UV-irradiation treatment, expression level of 18S rRNA… CONTINUE READING
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