Normal reproductive capacity of heifers that originated from in vitro fertilized embryos cultured with an antiviral compound.

@article{Givens2009NormalRC,
  title={Normal reproductive capacity of heifers that originated from in vitro fertilized embryos cultured with an antiviral compound.},
  author={M. Daniel Givens and Mylissa S D Marley and Kay P. Riddell and Patricia K. Galik and David A. Stringfellow},
  journal={Animal reproduction science},
  year={2009},
  volume={113 1-4},
  pages={
          283-6
        }
}
Bovine viral diarrhea virus (BVDV) can associate with in vitro fertilized (IVF) bovine embryos despite washing and trypsin treatment. An antiviral compound, DB606 (2-(4-[2-imidazolinyl]phenyl)-5-(4-methoxyphenyl)furan), inhibits the replication of BVDV in bovine uterine tubal epithelial cells, Madin Darby bovine kidney cells, and fetal fibroblast cells. As well, DB606 in in vitro culture medium does not affect embryonic development. Antiviral-treated-IVF embryos placed into recipients developed… 
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References

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Normal calves produced after transfer of in vitro fertilized embryos cultured with an antiviral compound.
TLDR
Evaluating the capability of IVF embryos to develop into normal, weaned calves after exposure to antiviral concentrations of DB606 during IVC concluded that bovine embryo cultures can be safely supplemented with antiviral Concentrations DB606; addition ofDB606 agent might prevent viral transmission if BVDV were inadvertently introduced into the embryo culture system.
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TLDR
It appears that BVDV-type II has the ability to be transferred with oocytes through the IVF system, resulting in infectious embryos with normal morphological appearance which may have a potential for disease transmission.
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TLDR
This study examined if noncytopathic BVDV-infected uterine tubal cells in IVF and IVC systems affected the rate of cleavage and development, and if either degenerated ova or embryos produced in the presence of the infected cells had virus associated with them after washing.
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TLDR
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TLDR
Preliminary results indicated that bovine embryo cultures might be safely supplemented with effective concentrations of an antiviral agent.
WASHING OR WASHING AND TRYPSIN TREATMENT IS INEFFECTIVE FOR REMOVAL OF NONCYTOPATHIC BOVINE VIRAL DIARRHEA VIRUS FROM BOVINE OOCYTES OR EMBRYOS AFTER EXPERIMENTAL VIRAL CONTAMINATION OF AN IN VITRO FERTILIZATION SYSTEM
TLDR
It is shown that under in vitro conditions, BVDV may be associated with groups of unwashed and washed oocytes and embryos, and may persist through the IVF process, and the standard washing procedure with or without trypsin treatment is not fully effective in rendering IVF embryos free of BVDv.
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TLDR
The study shows that when semen containing BVDV at the level of 105 - 106TCID50/ml) is used forIVF, the virus is not completely removed by any of the simple physical methods commonly used to prepare sperm for IVF.
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TLDR
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TLDR
Ulasonography makes it possible to diagnose LDA accurately in dubious cases and can replace exploratory laparotomy, which has been recommended as a last diagnostic resort (Dirksen 1990).
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TLDR
Heat inactivation of serum at 56 C for 30 minutes was found to be an unreliable method of eliminating BVDV from sera.
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