Normal Phototransduction in Drosophila Photoreceptors Lacking an InsP3 Receptor Gene

@article{Raghu2000NormalPI,
  title={Normal Phototransduction in Drosophila Photoreceptors Lacking an InsP3 Receptor Gene},
  author={Padinjat Raghu and Nansi Jo Colley and Rebecca Webel and Tracy James and Gaiti Hasan and Michal Danin and Zvi Selinger and Roger C. Hardie},
  journal={Molecular and Cellular Neuroscience},
  year={2000},
  volume={15},
  pages={429-445}
}
The Drosophila light-sensitive channels TRP and TRPL are prototypical members of an ion channel family responsible for a variety of receptor-mediated Ca(2+) influx phenomena, including store-operated calcium influx. While phospholipase Cbeta is essential, downstream events leading to TRP and TRPL activation remain unclear. We investigated the role of the InsP(3) receptor (InsP(3)R) by generating mosaic eyes homozygous for a deficiency of the only known InsP(3)R gene in Drosophila. Absence of… 
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128 Citations
Highly Influential Citations
3
Background Citations
50
Methods Citations
9
Results Citations
5

128 Citations

Phototransduction in Drosophila Is Compromised by Gal4 Expression but not by InsP3 Receptor Knockdown or Mutation
TLDR
Several GMRGal4 phenotypes suggestive of compromised development are described, including reductions in sensitivity, dark noise, potassium currents, and cell size and capacitance, as well as extreme variations in sensitivity between cells.
Phototransduction mechanisms in Drosophila microvillar photoreceptors
TLDR
Ca2+ influx via TRP channels is essential for rapid kinetics, amplification, and light adaptation and mediates both positive and negative feedback via multiple downstream targets, including the channels, rhodopsin, and PLC.
Mechanisms of Light Adaptation in Drosophila Photoreceptors
Functional INAD complexes are required to mediate degeneration in photoreceptors of the Drosophila rdgA mutant
TLDR
The results demonstrate a previously unknown requirement for a balance of PLCβ and DGK activity for retinal degeneration in rdgA and suggest a key role for the lipid products of phosphoinositide hydrolysis in the activation of TRP channels in vivo.
Calcium Influx via TRP Channels Is Required to Maintain PIP2 Levels in Drosophila Photoreceptors
Activation of TRP Channels by Protons and Phosphoinositide Depletion in Drosophila Photoreceptors
1.05 – Phototransduction in Microvillar Photoreceptors of Drosophila and Other Invertebrates
Phototransduction and retinal degeneration in Drosophila
TLDR
Drosophila visual transduction appears to have particular relevance to the cascade in the intrinsically photosensitive retinal ganglion cells in mammals, as the photoresponse in these latter cells appears to operate through a remarkably similar mechanism.
A Common Mechanism Underlies Vertebrate Calcium Signaling andDrosophila Phototransduction
TLDR
The results indicate an important link in the coupling mechanism of vertebrate store-operated channels and Drosophila TRP channels, which involves the InsP3 branch of the inositol lipid-signaling pathway.
TRP, TRPL and Cacophony Channels Mediate Ca2+ Influx and Exocytosis in Photoreceptors Axons in Drosophila
TLDR
New light is shed on the mechanism of neurotransmitter release in tonic synapses of non-spiking neurons in Drosophila by studying the distribution and physiological properties of TRP and TRPL in the lamina of the visual system.
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References

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TLDR
The results indicate that the response inactivation in trp cells results from Ca2+ deficiency rather than from Ca(2+)-dependent negative feedback, and indicate that there is light-induced release of Ca 2+ from intracellular stores.
The light response of drosophila photoreceptors is accompanied by an increase in cellular calcium: Effects of specific mutations
The Drosophila Light-Activated Conductance Is Composed of the Two Channels TRP and TRPL
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TLDR
It is suggested that another polyunsaturated fatty acid, such as linolenic acid, may be a messenger of excitation in Drosophila photoreceptors, as four different lipoxygenase inhibitors, which might be expected to lead to build-up of endogenous fatty acids, also activate native TRP and TRPL channels in intact photoresceptors.
Photolysis of caged Ca2+ facilitates and inactivates but does not directly excite light-sensitive channels in Drosophila photoreceptors
  • R. Hardie
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1995
TLDR
Caged Ca2+ failed to activate any channels in Drosophila photoreceptors but profoundly affected the light-dependent channels once they have been activated.
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