Nonvirally Modified Autologous Primary Hepatocytes Correct Diabetes and Prevent Target Organ Injury in a Large Preclinical Model

@article{Chen2008NonvirallyMA,
  title={Nonvirally Modified Autologous Primary Hepatocytes Correct Diabetes and Prevent Target Organ Injury in a Large Preclinical Model},
  author={Nelson K. F. Chen and Jen San Wong and Irene H. C. Kee and Siang Hui Lai and C. H. Thng and Wai Har Ng and Robert T. H. Ng and Soo Yong Tan and Shu Yen Lee and Mark Tan and Jaichandran Sivalingam and Pierce K. H. Chow and Oi Lian Kon},
  journal={PLoS ONE},
  year={2008},
  volume={3},
  pages={313 - 316}
}
BACKGROUND Current gene- and cell-based therapies have significant limitations which impede widespread clinical application. Taking diabetes mellitus as a paradigm, we have sought to overcome these limitations by ex vivo electrotransfer of a nonviral insulin expression vector into primary hepatocytes followed by immediate autologous reimplantation in a preclinical model of diabetes. METHODS AND RESULTS In a single 3-hour procedure, hepatocytes were isolated from a surgically resected liver… CONTINUE READING
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Human hepatocyte

  • RA Fisher, SC Strom
  • TRANSPLANTATION
  • 2006

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