Nonviral vector-mediated thymidine kinase gene transfer and ganciclovir treatment in leiomyoma cells.


OBJECTIVE To test the hypotheses that ganciclovir is cytotoxic to leiomyoma cells transfected with herpes simplex virus thymidine kinase and that estrogen modulates the responsiveness of tumor cells to this gene therapy approach. METHODS Human and rat cultured uterine leiomyoma cells were transfected with plasmids encoding the beta-galactosidase gene… (More)