Nontoxic genetic engineering of mesenchymal stem cells using serum-compatible pullulan-spermine/DNA anioplexes.

@article{Thakor2011NontoxicGE,
  title={Nontoxic genetic engineering of mesenchymal stem cells using serum-compatible pullulan-spermine/DNA anioplexes.},
  author={Devang K Thakor and Yang Dong Teng and Hideaki Obata and Kentaro Nagane and Shigeru Saito and Yasuhiko Tabata},
  journal={Tissue engineering. Part C, Methods},
  year={2011},
  volume={17 2},
  pages={131-44}
}
Genetic modification of stem cells could be applied to initiate/enhance their secretion of therapeutic molecules, alter their biological properties, or label them for in vivo tracking. We recently developed a negatively charged gene carrier ("anioplex") based on pullulan-spermine, a conjugate prepared from a natural polysaccharide and polyamine. In rat mesenchymal stem cells (MSCs), anioplex-derived reporter gene activity was comparable to or exceeded that obtained using a commercial cationic… CONTINUE READING

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