Nonspecific cytotoxic cells in fish (Ictalurus punctatus). II. Parameters of target cell lysis and specificity.

@article{Evans1984NonspecificCC,
  title={Nonspecific cytotoxic cells in fish (Ictalurus punctatus). II. Parameters of target cell lysis and specificity.},
  author={Donald Lee Evans and S S Graves and D A Cobb and D. L. Dawe},
  journal={Developmental and comparative immunology},
  year={1984},
  volume={8 2},
  pages={303-12}
}
Fish nonspecific cytotoxic cells (NCC) lyse various transformed human B-cells (NC-37, P3HR-1) and erythroblastoid cells (K562) as well as mouse YAC-1 and P815 cells. Highest NCC activity was found in the anterior (head) kidney, but spleen cells and peripheral blood leucocytes (PBL) also demonstrated cytolytic abilities. Lysis of chromium-51 labeled target cells occurred rapidly and optimum cytolysis developed at either 16 degrees C or 26 degrees C incubation temperatures. Preincubation at… CONTINUE READING