Nonspecific DNA binding of genome-regulating proteins as a biological control mechanism: measurement of DNA-bound Escherichia coli lac repressor in vivo.

@article{KaoHuang1977NonspecificDB,
  title={Nonspecific DNA binding of genome-regulating proteins as a biological control mechanism: measurement of DNA-bound Escherichia coli lac repressor in vivo.},
  author={Y Kao-Huang and A. Revzin and Andrew P. Butler and Patricia O'conner and D W Noble and Peter H. von Hippel},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1977},
  volume={74 10},
  pages={4228-32}
}
Binding of genome regulatory proteins to nonspecific DNA sites may play an important role in controlling the thermodynamics and kinetics of the interactions of these proteins with their specific target DNA sequences. An estimate of the fraction of Escherichia coli lac repressor molecules bound in vivo to the operator region and to nonoperator sites on the E. coli chromosome is derived by measurement of the distribution of repressor between a minicell-producing E. coli strain (P678-54) and the… CONTINUE READING
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