Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA.

@article{DiMaio1986NonsenseMI,
  title={Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA.},
  author={Daniel DiMaio},
  journal={Journal of virology},
  year={1986},
  volume={57 2},
  pages={475-80}
}
Oligonucleotide-directed mutagenesis was used to construct a nonsense mutation in open reading frame (ORF) E2 of bovine papillomavirus DNA. A single base substitution mutation was constructed which converted a TAC codon into a TAG amber stop codon at a position in the ORF that did not overlap with any other viral ORFs. Full-length viral DNA containing the mutation induced only approximately 2% of the transformed foci of mouse C127 cells that were induced by wild-type DNA. In a different… CONTINUE READING