Nonresonant femtosecond laser vaporization of aqueous protein preserves folded structure.

@article{Brady2011NonresonantFL,
  title={Nonresonant femtosecond laser vaporization of aqueous protein preserves folded structure.},
  author={John J. Brady and Elizabeth J Judge and Robert J Levis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 30},
  pages={12217-22}
}
Femtosecond laser vaporization-based mass spectrometry can be used to measure protein conformation in vitro at atmospheric pressure. Cytochrome c and lysozyme are vaporized from the condensed phase into the gas phase intact when exposed to an intense (10(13) W/cm(2)), nonresonant (800 nm), ultrafast (75 fs) laser pulse. Electrospray postionization time-of-flight mass spectrometry reveals that the vaporized protein maintains the solution-phase conformation through measurement of the charge-state… CONTINUE READING
Highly Cited
This paper has 22 citations. REVIEW CITATIONS
Related Discussions
This paper has been referenced on Twitter 1 time. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

Do charge state signatures guarantee protein conformations?

Journal of the American Society for Mass Spectrometry • 2012
View 3 Excerpts
Highly Influenced

High repetition-rate, pulse-burst assisted desorption, electrospray post-ionization mass spectrometry

2014 Conference on Lasers and Electro-Optics (CLEO) - Laser Science to Photonic Applications • 2014
View 2 Excerpts

References

Publications referenced by this paper.
Showing 1-10 of 64 references

Effects of matrix , electrospray solution , and laser light on the desorption and ionization mechanisms in electrosprayassisted laser desorption ionization mass spectrometry

Huang M-Z, Jhang S-S, Cheng C-N, Cheng S-C, J Shiea
Analyst • 2010

Effects of matrix, electrospray solution, and laser light on the desorption and ionization mechanisms in electrospray-assisted laser desorption ionization mass spectrometry. Analyst 135:759–766

Huang M-Z, Jhang S-S, Cheng C-N, Cheng S-C, J Shiea
2010
View 1 Excerpt

Similar Papers

Loading similar papers…