Nonradioactive detection of retroviral-associated RNase H activity in a microplate-based, high-throughput format.

@article{McLellan2002NonradioactiveDO,
  title={Nonradioactive detection of retroviral-associated RNase H activity in a microplate-based, high-throughput format.},
  author={N. Mason McLellan and Xiaomin Wei and Bruno Marchand and MarkA. Wainberg and Matthias G{\"o}tte},
  journal={BioTechniques},
  year={2002},
  volume={33 2},
  pages={
          424-9
        }
}
None of the available antiretroviral drugs that are currently used in the clinic to treat infection with HIV-1 is directed against the RNase H active site of the reverse transcriptase. Here we developed a nonradioactive, 96-well plate assay designed to be used for high-throughput screening of compounds capable of inhibiting the RNase H activity of HIV-1 reverse transcriptase. We employed a tRNA as substrate that was labeled with digoxygenin-modified reporter residues. The labeled tRNA was… CONTINUE READING

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