Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model.

@article{Bragonzi2005NonmucoidPA,
  title={Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model.},
  author={Alessandra Bragonzi and Dieter Worlitzsch and Gerald B Pier and Petra Timpert and M. D. Ulrich and Morten Hentzer and Jens Bo Andersen and Michael C Givskov and Massimo Conese and Gerd Doring},
  journal={The Journal of infectious diseases},
  year={2005},
  volume={192 3},
  pages={
          410-9
        }
}
BACKGROUND In patients with cystic fibrosis (CF), lung infection with mucoid Pseudomonas aeruginosa strains overexpressing the exopolysaccaride alginate is preceded by colonization with nonmucoid strains. We investigated the kinetics, impact of environmental signals, and genetics of P. aeruginosa alginate expression in a mouse model and in patients with CF. METHODS Using indirect immunofluorescence, microarray technology and real-time reverse-transcription polymerase chain reaction, we… CONTINUE READING
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