Noninvasive imaging of bone-specific collagen I expression in a luciferase transgenic mouse model.

Abstract

Luciferase transgenic mice are a very promising tool for noninvasive, quantitative, and longitudinal evaluation of gene expression. The aim of this study was to validate the Col(I)-Luc transgenic mouse model in which the luciferase gene is driven by bone-specific regulatory elements from the mouse collagen α1(I) gene for bioluminescent imaging of bone development and remodeling. We observed strong luciferase activity in skeletal tissues of Col(I)-Luc mice, and observed that the light intensity declined with postnatal bone development. Luciferase activity was enhanced in a tail bone repair model and we were able to monitor the process of ectopic bone formation induced by recombinant human bone morphogenetic protein 2 using bioluminescent imaging. We conclude that Col(I)-Luc transgenic mice can be applied in the field of bone tissue engineering for monitoring bone repair processes and for investigating osteoinductive molecules or scaffolds.

DOI: 10.1089/ten.TEC.2009.0594

Cite this paper

@article{Liu2010NoninvasiveIO, title={Noninvasive imaging of bone-specific collagen I expression in a luciferase transgenic mouse model.}, author={Jun Liu and Roel J. Arends and Anton C. M. Martens and Maria H C den Brok-Bardoel and Marian G H Scheepers and Clemens A. van Blitterswijk and Jan Freark de Boer}, journal={Tissue engineering. Part C, Methods}, year={2010}, volume={16 6}, pages={1297-304} }