Noninvasive genetic tracking of the endangered Pyrenean brown bear population

  title={Noninvasive genetic tracking of the endangered Pyrenean brown bear population},
  author={Pierre Taberlet and Jean-Jacques Camarra and S. Griffin and E. Uhr{\`e}s and Oliver Hanotte and Lisette P. Waits and C Dubois-Paganon and Terry A. Burke and J. -M. Bouvet},
  journal={Molecular Ecology},
Pyrenean brown bears Ursus arctos are threatened with extinction. Management efforts to preserve this population require a comprehensive knowledge of the number and sex of the remaining individuals and their respective home ranges. This goal has been achieved using a combination of noninvasive genetic sampling of hair and faeces collected in the field and corresponding track size data. Genotypic data were collected at 24 microsatellite loci using a rigorous multiple‐tubes approach to avoid… 

The power of genetic monitoring for studying demography, ecology and genetics of a reintroduced brown bear population

This study provides a model for other reintroduction programmes by demonstrating how genetic monitoring can be implemented to uncover aspects of the demography, ecology and genetics of small and reintroduced populations that will advance the understanding of the processes influencing their viability, evolution, and successful restoration.

Genetics and conservation of European brown bears Ursus arctos

The European brown bear has served, and will continue to serve, as a model for the development of methods, analyses and hypotheses in conservation genetics, and many non-invasive genetic methods have been used for individual identification, censusing populations, monitoring migration and gene flow, and testing methods that are easier to use in endangered populations and over large areas.

Non-invasive sampling of Snow Leopards (Uncia uncia) in Phu valley, Nepal

iii Abstract Snow leopards (Uncia uncia) inhabit the remote mountain ranges of the Himalayas, and are currently listed as Endangered. Due to their inaccessible habitat and sparse distribution it is

Sex-biased natal dispersal in Hokkaido brown bears revealed through mitochondrial DNA analysis

The study revealed that mtDNA haplotype distribution has been maintained by female philopatry, and that bears exhibit male-biased dispersal, and the lower peninsula appears to act as a contact zone between the peninsula and mainland Hokkaido, which is important for maintaining genetic diversity.

Non‐invasive genetic censusing and monitoring of primate populations

This work has shown that the confidence interval of point estimates includes the true population size when assumptions of the models are met, and therefore this range of population size minima and maxima should be emphasized in population monitoring studies.

Noninvasive monitoring of wolves at the edge of their distribution and the cost of their conservation

Large predators are recolonizing areas in industrialized countries, where they have been absent for decades or centuries. As they reach these areas, the predators often encounter unwary livestock and

Impacts of sampling location within a faeces on DNA quality in two carnivore species

The sampling location within a faeces should be carefully considered and reported as it can directly influence laboratory costs and efficiency, as well as the ability to obtain reliable genotypes.

Possible conservation units of the sun bear (Helarctos malayanus) in Sarawak based on variation of mtDNA control region.

The mitochondrial DNA control region of the sun bear was sequenced using 21 DNA samples collected from confiscated sun bears to identify conservation units, such as evolutionarily significant units and management units, in Sarawak, Borneo Island, indicating the presence of at least two lineages.

Determinants of male reproductive success in American black bears

The observed peak in reproductive success occurred at roughly the same intermediate age as milder peaks in body size characteristics and frequency of bear-inflicted wounds, suggesting body size and fighting might be important for mating.



Microsatellite analysis of paternity and reproduction in Arctic grizzly bears.

Analysis of microsatellite loci of 30 grizzly bear family groups demonstrate that each cub in a litter can be sired independently, and estimates of maximum reproductive success for males are derived from an Arctic population in northwestern Alaska that is minimally affected by human activities.

Conservation genetics of the European brown bear ‐ a study using excremental PCR of nuclear and mitochondrial sequences

It is shown that the Brenta population is monomorphic for one mitochondrial lineage and that female as well as male bears exist in the area and the implications for the management of brown bears in the BrentA and elsewhere in Europe are discussed.

Microsatellite analysis of population structure in Canadian polar bears

Using eight hypervariable microsatellite loci to study the genetic relationships between four Canadian polar bear populations indicates that gene flow between local populations is restricted despite the long‐distance seasonal movements undertaken by polar bears.

Mitochondrial DNA polymorphism, phylogeography, and conservation genetics of the brown bear Ursus arctos in Europe

  • P. TaberletJ. Bouvet
  • Biology
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 1994
This study demonstrates the relevance of the molecular phylogeographic approach to the identification of conservation units of the brown bear and deduces the potential conservation units from these results.

Movements of Yellowstone grizzly bears

Paternity exclusion in a community of wild chimpanzees using hypervariable simple sequence repeats

The use of hypervariable simple sequence repeat (SSR) nuclear loci to study paternity in a community of wild chimpanzees in Gombe National Park, Tanzania allows the probable identification of the previously undetermined father; these are the first such determinations for free‐ranging chimpanzees, and the first based on non‐invasive sampling.

Sexing free‐ranging brown bears Ursus arctos using hairs found in the field

As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested

Kin selection, social structure, gene flow, and the evolution of chimpanzees.

Sequence variation patterns at two mitochondrial loci indicate historically high long-distance gene flow and clarify the relationships among three allopatric subspecies and imply that P. t.

Amplification of hypervariable simple sequence repeats (microsatellites) from excremental DNA of wild living bonobos (Pan paniscus)

We show that nuclear DNA extracted from faeces of free living bonobos (Pan paniscus) can be used to amplify hypervariable simple sequence repeats, which can be used for paternity analysis and kinship

Primers for the differential amplification of the sex‐determining region Y gene in a range of mammal species

Primers to SRY allow the amplification of a 216bp product from the SRY gene in representatives of six of the eight mammalian orders tested and are designed to complement part of the conserved HMG-box region common to members of the SOX gene family.