Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysis of human urine.

@article{Burgess2003NoninvasiveEO,
  title={Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysis of human urine.},
  author={Shawn C. Burgess and B. Weis and J. Gwynfryn Jones and Erin Smith and Matthew E Merritt and David P. Margolis and A Dean Sherry and Craig Malloy},
  journal={Analytical biochemistry},
  year={2003},
  volume={312 2},
  pages={228-34}
}
Mammalian liver disposes of acetaminophen and other ingested xenobiotics by forming soluble glucuronides that are subsequently removed via renal filtration. When given in combination with the stable isotopes 2H and 13C, the glucuronide of acetaminophen isolated from urine provides a convenient "chemical biopsy" for evaluating intermediary metabolism in the liver. Here, we describe isolation and purification of urinary acetaminophen glucuronide and its conversion to monoacetone glucose (MAG… CONTINUE READING