Noninvasive, repetitive, quantitative measurement of gene expression from a bicistronic message by positron emission tomography, following gene transfer with adenovirus.

@article{Liang2002NoninvasiveRQ,
  title={Noninvasive, repetitive, quantitative measurement of gene expression from a bicistronic message by positron emission tomography, following gene transfer with adenovirus.},
  author={Qianwa Liang and Jeff Gotts and Nagichettiar Satyamurthy and Jorge R. Barrio and Michael E. Phelps and Sanjiv Sam Gambhir and Harvey R. Herschman},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2002},
  volume={6 1},
  pages={73-82}
}
Gene therapy protocols are hampered by the inability to monitor the location, magnitude, and duration of ectopic gene expression following DNA delivery. Consequently, it is difficult to establish quantitative correlations and/or causal relationships between therapeutic gene expression and phenotypic responses in treated individuals. One approach to monitor "therapeutic gene" expression indirectly is to incorporate reporter genes that can be imaged in vivo into bicistronic transcription units… CONTINUE READING