Noninvasive, quantitative imaging in living animals of a mutant dopamine D2 receptor reporter gene in which ligand binding is uncoupled from signal transduction

@article{Liang2001NoninvasiveQI,
  title={Noninvasive, quantitative imaging in living animals of a mutant dopamine D2 receptor reporter gene in which ligand binding is uncoupled from signal transduction},
  author={Qianwa Liang and Nagichettiar Satyamurthy and J G Barrio and Tatsushi Toyokuni and M C Phelps and S. S. Gulu Gambhir and HR Herschman},
  journal={Gene Therapy},
  year={2001},
  volume={8},
  pages={1490-1498}
}
The dopamine D2 receptor (D2R) has been used in adenoviral delivery systems and in tumor cell xenografts as an in vivo reporter gene. D2R reporter gene expression has been non-invasively, repetitively and quantitatively imaged by positron emission tomography (PET), following systemic injection of a positron-labeled ligand (3-(2′-[18F]-fluoroethyl)-spiperone; FESP) and subsequent D2R-dependent sequestration. However, dopamine binding to the D2R can modulate cyclic AMP levels. For optimal… CONTINUE READING