Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data

@article{Chin2013NonhybridFM,
  title={Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data},
  author={Chen-Shan Chin and David H. Alexander and Patrick Marks and Aaron A. Klammer and James R. Drake and Cheryl Heiner and Alicia Clum and Alex Copeland and John Huddleston and Evan E. Eichler and Stephen P. Turner and Jonas Korlach},
  journal={Nature Methods},
  year={2013},
  volume={10},
  pages={563-569}
}
We present a hierarchical genome-assembly process (HGAP) for high-quality de novo microbial genome assemblies using only a single, long-insert shotgun DNA library in conjunction with Single Molecule, Real-Time (SMRT) DNA sequencing. Our method uses the longest reads as seeds to recruit all other reads for construction of highly accurate preassembled reads through a directed acyclic graph–based consensus procedure, which we follow with assembly using off-the-shelf long-read assemblers. In… CONTINUE READING
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