Nonessential active site residues modulate selenosubtilisin's kinetic mechanism.

Abstract

Selenosubtilisin, a semisynthetic enzyme produced by chemical modification of subtilisin's catalytic serine, mimics the antioxidant enzyme glutathione peroxidase, catalyzing the reduction of hydroperoxides by 3-carboxy-4-nitrobenzenethiol. In analogy with the unmodified protease, selenosubtilisins derived from distantly related subtilisin templates exhibit… (More)

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