Nonenzymatic glycation of human platelet membrane proteins in vitro and in vivo.

@article{Sampietro1986NonenzymaticGO,
  title={Nonenzymatic glycation of human platelet membrane proteins in vitro and in vivo.},
  author={Tiziana Sampietro and Santiago Lenzi and Paolo Cecchetti and Ottavio Giampietro and Lorella Cruschelli and Renzo Navalesi},
  journal={Clinical chemistry},
  year={1986},
  volume={32 7},
  pages={1328-31}
}
Human platelet membrane proteins (PMP), incubated in vitro in the presence of various concentrations of glucose, undergo nonenzymatic glycation, as evidenced by incorporation of [3-3H]glucose radioactivity into the acid-precipitable fraction. The time course of the reaction is linear for the first hours, and the rate of glycation depends on the glucose concentration in the medium: at a glucose concentration of 80 mmol/L, up to 60 nmol of glucose is bound per milligram of PMP. The ketoaminic… CONTINUE READING