Nonenzymatic glucosylation of human serum albumin and its influence on binding capacity of sulfonylureas.

@article{Tsuchiya1984NonenzymaticGO,
  title={Nonenzymatic glucosylation of human serum albumin and its influence on binding capacity of sulfonylureas.},
  author={Seishi Tsuchiya and T Sakurai and S Sekiguchi},
  journal={Biochemical pharmacology},
  year={1984},
  volume={33 19},
  pages={
          2967-71
        }
}
To estimate the functional change occurring in human serum albumin by nonenzymatic glucosylation, glucosylated human serum albumin was prepared by in vitro incubation with glucose. The rate of glucosylation proceeded as a first-order reaction. The binding of sulfonylureas to serum albumin was determined by equilibrium gel filtration. Through this method, it was possible to estimate the binding capacity of a low water solubility drug with a high affinity to protein. The amounts of the… 
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