Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring.

Abstract

Here, we report a method for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and mRNA from a variety of cell types. Cytosolic contents were repeatedly sampled from the same cell or population of cells for more than 5 d through a cell-culture substrate, incorporating hollow 150-nm-diameter nanostraws (NS) within… (More)
DOI: 10.1073/pnas.1615375114

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