Nondestructive detection of glutamate by 1H nuclear magnetic resonance spectroscopy in cortical brain slices from the guinea pig: evidence for changes in detectability during severe anoxic insults.

@article{Kauppinen1991NondestructiveDO,
  title={Nondestructive detection of glutamate by 1H nuclear magnetic resonance spectroscopy in cortical brain slices from the guinea pig: evidence for changes in detectability during severe anoxic insults.},
  author={Risto A. Kauppinen and Stephen Ross Williams},
  journal={Journal of neurochemistry},
  year={1991},
  volume={57 4},
  pages={1136-44}
}
31P and 1H nuclear magnetic resonance spectroscopy (NMR) was used to study the metabolism of intact superfused cortical brain slices during normoxia and anoxia. Attention was focused on quantification of 1H NMR-detected glutamate by a water-suppressed spin-echo method, using N-acetyl aspartate as an internal concentration reference. To quantify the 1H NMR… CONTINUE READING