Non starch polysaccharide hydrolyzing enzymes as feed additives: detection of enzyme activities and problems encountered with quantitative determination in complex samples.

@article{Vahjen1997NonSP,
  title={Non starch polysaccharide hydrolyzing enzymes as feed additives: detection of enzyme activities and problems encountered with quantitative determination in complex samples.},
  author={Wilfried Vahjen and Konieczny Glaeser and M Froeck and Ortwin Simon},
  journal={Archiv fur Tierernahrung},
  year={1997},
  volume={50 4},
  pages={331-45}
}
Chromogenic substrates, an agar diffusion assay and viscosity reduction were used to estimate beta-glucanase and xylanase activities in water soluble extracts of different feedstuffs and digesta supernatants. The dinitrosalicylic acid reducing sugar method was employed to calibrate results from different methods based on international units (IU, glucose equivalents). The detection of dye release from chromogenic substrates was a suitable method, allowing the detection of 0.05 IU of enzyme… CONTINUE READING
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