Non-radioactive direct sequencing of PCR products amplified from neuropathological specimens.

Abstract

We have developed a simple, rapid and relatively inexpensive protocol for direct non-isotopic cycle sequencing of DNA amplified using the polymerase chain reaction (PCR). PCR is performed on routine and archival neuropathological tissue. For sequencing, a 5'-digoxigenin end-labelled oligonucleotide primer is annealed and extended during thermal cycling… (More)

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Cite this paper

@article{Ksel1993NonradioactiveDS, title={Non-radioactive direct sequencing of PCR products amplified from neuropathological specimens.}, author={Siegfried K{\"{o}sel and Manuel Graeber}, journal={Brain pathology}, year={1993}, volume={3 4}, pages={421-4} }