Non-methylated Genomic Sites Coincidence Cloning (NGSCC): an approach to large scale analysis of hypomethylated CpG patterns at predetermined genomic loci

@article{Azhikina2003NonmethylatedGS,
  title={Non-methylated Genomic Sites Coincidence Cloning (NGSCC): an approach to large scale analysis of hypomethylated CpG patterns at predetermined genomic loci},
  author={Tatyana Azhikina and Ildar V Gainetdinov and Yu. V. Skvortsova and Anastasia Batrak and N. P. Dmitrieva and Eugene D. Sverdlov},
  journal={Molecular Genetics and Genomics},
  year={2003},
  volume={271},
  pages={22-32}
}
We have developed a new approach to the analysis of hypomethylated CpG patterns within predetermined, megabase long, genome regions. The approach, which we term Non-methylated Genomic Sites Coincidence Cloning (NGSCC), includes three main steps. First, total genomic DNA is digested with a methylation sensitive restriction endonuclease, such as Hpa II or Hha I. Then the fragments corresponding to the genomic area of interest are selected. To this end the fragmented genome DNA is hybridized with… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

Epigenetics Protocols

Methods in Molecular Biology • 2011

Epigenetics--a helpful tool to better understand processes in clinical nephrology?

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association • 2008
View 1 Excerpt

References

Publications referenced by this paper.
Showing 1-10 of 40 references

Methylation matters: modeling a manageable genome.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research • 2002
View 1 Excerpt

Similar Papers

Loading similar papers…