Non-isotopic hybridization assays for bacterial DNA samples.

Abstract

The background problem associated with the use of streptavidin in detecting biotin-labelled probes hybridized to DNA in crude bacterial extracts has been investigated. We have found that streptavidin binds specifically to a limited number of polypeptides which are difficult to remove by rapid extraction processes. Altering the hybridization and detection… (More)

Topics

Cite this paper

@article{Kennedy1989NonisotopicHA, title={Non-isotopic hybridization assays for bacterial DNA samples.}, author={K. E. Kennedy and Spyros Daskalakis and Lee Davies and Peter Zwadyk}, journal={Molecular and cellular probes}, year={1989}, volume={3 2}, pages={167-77} }